Automated DNA Image Ploidy System
DNA Ploidy is becoming an accepted prognostic marker. The DNA Ploidy test alone can be used to obtain prognostic information to determine treatment decisions in prostatic, urinary bladder, ovarian, endometrial and renal cell carcinoma among others. The test can also be an additive prognostic marker. DNA Ploidy provides a cost effective and objective way to assess the progression, make treatment decisions and to stratify patient groups.
Video showing system in operation click here.
Why Use Automated DNA Image Ploidy?
- Measurement of DNA content within a cell nuclei on a monolayer preparation, selecting only desired tissue in the analysis
Only the epithelial cells are included in the analyis so higher accuracy compared to other methods
- High levels of automation means that thousands of nuclei can be included in measurements
System Description
- The system is based on a research microscope and a high quality digital camera. It includes software and hardware to capture images from the monolayer and to produce a histogram of the DNA content of the cell nuclei enabling a DNA Ploidy classification to be made
- Automated Cell Capture- Place the slide on the microscope, check the microscope setup and press go
- Automated Cell Type Classification - Nuclei type are classified automatically
- Automated Guidance on DNA Ploidy Classification - The system, based on published rules on DNA ploidy classification suggests a histogram classification
Selected Publications on DNA Ploidy
- Baak JP, Snijders W, van Diermen B, van Diest PJ, Diepenhorst FW, Benraadt J. Prospective multicenter validation confirms the prognostic superiority of the endometrial carcinoma prognostic index in international Federation of gynecology and obstetrics stage 1 and 2 endometrial carcinoma.J Clin Oncol. 2003 Nov 15;21(22):4214-21
- Choma D, Daures JP, Quantin X, and Pujol JL. Aneuploidy and prognosis of non-small-cell lung cancer: a meta-analysis of published data. Br J Cancer, 85: 14-22, 2001
- Gazic B, Pizem J, Bracko M, Cufer T, Borstnar S, Pohar-Marinsek Z, and Us-Krasovec M. S-phase fraction determined on fine needle aspirates is an independent prognostic factor in breast cancer - a multivariate study of 770 patients. Cytopathology, 19: 294-302, 2008
- Pretorius ME, Waehre H, Abeler VM, Davidson B, Vlatkovic L, Lothe RA, Giercksky KE, Danielsen HE. Large scale genomic instability as an additive prognostic marker in early prostate cancer.Cell Oncol. 2009;31(4):251-9
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